Transposable Elements in Maize the?Activator-Dissociation (Ac-Ds) System
نویسندگان
چکیده
منابع مشابه
Isolation of the transposable maize controlling elements Ac and Ds.
Restriction endonuclease fragments containing part of the Waxy (Wx) locus have been cloned from strains with insertion mutations at the locus caused by the controlling elements Activator (Ac) and Dissociation (Ds). Evidence is presented that the genetically defined Ac element corresponds to a 4.3 kb insertion, while the two Ds elements correspond to 4.1 kb and 2.0 kb insertions, all near the 3'...
متن کاملAnalysis of extrachromosomal Ac/Ds transposable elements.
The mechanism of transposition of the maize Ac/Ds elements is not well understood. The true transposition intermediates are not known and it has not been possible to distinguish between excision models involving 8-bp staggered cuts or 1-bp staggered cuts followed by hairpin formation. In this work, we have analyzed extrachromosomal excision products to gain insight into the excision mechanism. ...
متن کاملChromosome breakage by pairs of closely linked transposable elements of the Ac-Ds family in maize.
Chromosome breaks and hence chromosomal rearrangements often occur in maize stocks harboring transposable elements (TEs), yet it is not clear what types of TE structures promote breakage. We have shown previously that chromosomes containing a complex transposon structure consisting of an Ac (Activator) element closely linked in direct orientation to a terminally deleted or fractured Ac (fAc) el...
متن کاملMaize Transposable Elements Ac/Ds as Insertion Mutagenesis Tools in Candida albicans
In nonmodel systems, genetic research is often limited by the lack of techniques for the generation and identification of gene mutations. One approach to overcome this bottleneck is the application of transposons for gene tagging. We have established a two-element transposon tagging system, based on the transposable elements Activator (Ac)/Dissociation (Ds) from maize, for in vivo insertion mut...
متن کاملThe Activator/Dissociation (Ac/Ds) transposable elements comprise a two- component gene regulatory switch that controls endogenous gene expression in maize
The maize Activator/Dissociation (Ac/Ds) elements are able to replicate and transpose throughout the maize genome. Both elements preferentially insert into gene-rich regions altering the maize genome by creating unstable insertion alleles, stable derivative or excision alleles or by altering the spatial or temporal regulation of gene expression. Here, we characterize an Ac insertion in the 5' U...
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1984
ISSN: 0004-9417
DOI: 10.1071/bi9840307